Why do longer PCR primers have a higher annealing temperature and why do you get more specificity? - annealing temperature higher more specific
Please give a detailed answer if possible
Sunday, February 14, 2010
Annealing Temperature Higher More Specific Why Do Longer PCR Primers Have A Higher Annealing Temperature And Why Do You Get More Specificity?
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The longer the primer strongest interaction with the target DNA, since more hydrogen bonding occurs between the complementary bases. Increased specificity for the same reason. The longer the primer further information is stored in its sequence, more than able to recognize the correct sequence corresponding to the target DNA. Increase interaction associated with multiple primers led to greater stability at high temperatures, so that the higher annealing temperature.
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